目的 研究sparstolonin B(8,5'-dihydroxy-4-phenyl-5,2'-oxidoisocoumarin, SsnB)对脑出血(intracerebral haemorrhage, ICH)诱导的大鼠脑损伤的保护作用。方法 体外Transwell实验观察SsnB对血红蛋白(hemoglobin, Hb)诱导的小胶质细胞损伤神经元的影响;采用自体血注射脑出血模型,出血后的第1、3、5、7天观察SsnB对ICH大鼠神经功能缺损评分(neurological deficit score,NDS),脑组织含水量的影响,ELISA法检测ICH大鼠脑组织匀浆中炎性细胞因子白细胞介素(IL)-6、IL-1β和肿瘤坏死因子(TNF)-α的含量变化。结果 第1、3天,5和15 mg·kg-1SsnB都能减少ICH大鼠的NDS,降低ICH大鼠脑组织含水量,明显下调脑血肿周围组织中炎性细胞因子的含量。结论 SsnB对ICH大鼠脑损伤有保护作用,其机制可能与SsnB保护受损神经元,减轻脑出血大鼠神经功能缺损,降低脑组织含水量和炎症因子有关。
Abstract
OBJECTIVE To study the protective effects of sparstolonin B(8,5'-dihydroxy-4-phenyl-5,2'-oxidoisocoumarin,SsnB) on mouse with intracerebral haemorrhage(ICH). METHODS The effect of SsnB on neuronal cell death induced by Hb using a microglia-neuron transwell system was investigated. Autologous nonanticoagulated blood was collected from the tail artery of the mouse and then injected into the striatum using a syringe pump. Neurological deficit of the mouse with ICH was assessed 1, 3, 5 and 7 d after SsnB administration using modified neurological severity score system,and brain water content of the mouse cerebral tissues after ICH was measured at the same day. The concentrations of pro-inflammatory cytokines in perihematoma tissues, including interleukin-6, interleukin-1β and tumor necrosis factor-α, were measured by ELISA assay. RESULTS The RESULTS show that SsnB significantly reduced the neurological deficit scores and the brain water content, and inhibited the levels of IL-6, IL-1β and TNF-α at first day and third day after ICH. CONCLUSION SsnB can improve the brain injury in mouse induced by ICH. The mechanism may involve increased the neuronal survival rate and decreased expression of pro-inflammatory cytokines of mouse after ICH.
关键词
Sparstolonin B /
脑出血 /
神经元存活率 /
神经功能缺损评分 /
脑组织含水量 /
炎性因子
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Key words
sparstolonin B /
intracerebral hemorrhage /
neuronal survival rate /
neurological deficit score /
brain water content /
pro-inflammatory cytokines
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中图分类号：
R965
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参考文献
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脚注
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基金
国家自然科学基金资助项目(81173515);江苏省中药资源产业化过程协同创新中心重点项目(ZDXM-2-4);江苏省研究生培养创新工程
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